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To improve the efficacy and safety of ADA-SCID gene therapy (GT), we generated a self-inactivating lentiviral vector (LV) with a codon-optimized human cADA gene under the control of the short form elongation factor-1α promoter (LV EFS ADA).
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cultured with the EFS-ADA LV (Indiana University Vector Production Unit) for ~20 hours in the same complete medium 6at a cell concentration of 0.5 to 1 x 10 cells/ml. Following
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